Illumina/TruSeq DNA Methylation Kit

Illumina/TruSeq DNA Methylation Kit

Product Highlights:
Unlock small samples (50–100 ng DNA input)
CpG, CHH, & CHG regions included for comprehensive, whole-genome results
Fast protocol – five-hour method
Capture full sample diversity
Sequence the entire sample–no loss of information
The process of bisulfite treatment denatures genomic DNA into single stranded DNA. TruSeq DNA Methylation converts single stranded DNA into an Illumina sequencing library. All ssDNA fragments are captured into an Illumina sequencing library, therefore eliminating the loss of diversity associated with other methods.

Supported analysis in the cloud
TruSeq DNA Methylation libraries can be aligned to the human genome and compared for differential methylation with Illumina BaseSpace Apps MethylSeq and MethylKit. These applications are fully supported and were developed specifically for TruSeq DNA Methylation library preparation.

Example data sets for TruSeq DNA Methylation libraries, also available in BaseSpace Sequence Hub Data Central (use the “Methyl Seq” category filter), demonstrate unparalleled quality and seamless analysis.

Deep coverage of critical genomic regions
Depth of coverage is enhanced in genomic areas with BIOLOGical utility. TruSeq DNA Methylation captures full sample diversity of critical areas, including:

Coding region start and end for exons from the canonical transcript of protein coding genes for genes known to be involved in cancer, taken from SOMA and CRUK panels (as well as literature-derived cancer genes)
Genes defined by the American College of Medical Genetics as being medically relevant (ACMG genes)
Exonic coding regions from Ensemble 70
List of 100 promoters defined by the Broad Institute as being of high interest and difficult to sequence