用于Qubit熒光計(jì)的Qubit試劑盒，配合簡(jiǎn)單的混合讀取模式，均采用相同的操作方式。其中DNA和RNA分析試劑盒僅需孵育2分鐘，而蛋白分析試劑盒需要15分鐘 (圖 3)。 Qubit 4熒光計(jì)自動(dòng)保存您的數(shù)據(jù)，您可用USB數(shù)據(jù)線或U盤導(dǎo)出。 除了常規(guī)的 dsDNA，寡核苷酸，總RNA，小RNA和蛋白質(zhì)的分析試劑盒，在 Ion Personal Genome Machine(PGM) 測(cè)序儀上進(jìn)行測(cè)序之前，可使用專門的Ion Sphere Quality Control Kit在Qubit 4熒光計(jì)上進(jìn)行Ion Sphere顆粒質(zhì)量評(píng)估。

Product Line: Qubit™

Product Size: 100 assays

Invitrogen 1X dsDNA HS Assay Kit

Invitrogen 1X dsDNA HS Assay Kit

Green Features: Fewer resources used, Less waste

Shipping Condition: Room Temperature

內(nèi)容及儲(chǔ)存

Store in refrigerator at 2–8°C and protect from light.

The Qubit 1X dsDNA HS (High-Sensitivity) Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. The "1X" dsDNA assay kit provides reagent and buffer in a ready-to-use formulation. Simply add your sample (any volume between 1 µL and 20 µL is acceptable), add the Qubit 1X dsDNA HS reagent, and read the concentration using a Qubit 2.0, 3, 4, or Flex fluorometer. Common contaminants such as salts, free nucleotides, solvents, detergents, and protein are well-tolerated in the assay.

Notes:
1. The Qubit 1X dsDNA HS Assay works on Qubit 2.0, 3, 4, and Flex fluorometers. It will not work on the original Qubit 1.0 Fluorometer. Download the MyQubit .qbt file for the Qubit 2.0 or 3 fluorometer.
2. 500-mL thin-walled tubes displaying low auto-fluorescence (Cat. No. Q32856) are required but not included.