Illumina ForenSeq DNA Signature Prep Kit

Illumina ForenSeq DNA Signature Prep Kit

Library Preparation Kits

Product Highlights:

The ForenSeq DNA Signature Prep Kit is part of a complete, fully validated DNA-to-data solution designed for forensic genomics applications. It includes all reagents to prepare next-generation sequencing (NGS) libraries from forensic DNA samples.

Achieve high resolution and exceptional accuracy from as little as 1 ng of DNA—Even with complex mixtures or degraded DNA. This kit allows labs to:

Perform multiplexing and rapid sample processing

Access a wider range of informative single-nucleotide polymorphisms (SNPs) with a single kit

Analyze challenging samples

Multiplexing and rapid sample processing

The ForenSeq DNA Signature Prep Kit supports preparation of up to 96 libraries simultaneously using a simple plate-based format and standard lab equipment. Targeted primer mixes enable analysis of autosomal, Y- and X-chromosome short tandem repeat (STR) targets, and identity-informative SNPs, all in a single reaction.

Optionally, you can include biogeographical ancestry-informative SNPs (aiSNPs) and phenotypic-informative SNPs (piSNPs). This data can be critical in generating investigative leads from “no sUSPect” cases that have otherwise gone cold.

A wider range of informative SNPs with a single kit

The ForenSeq DNA Signature Prep Kit consolidates all autosomal STR Markers currently used around the world for casework and criminal DNA databases. This single, streamlined workflow eliminates the need to run multiple STR tests.

The kit delivers approximately 200 genetic Markers in a single test, removing the tradeoffs and risk imposed by technical limitations such as low DNA quantity. It also contains a dense set of identity informative single nucleotide polymorphism (iiSNP) Marker sets not routinely available with trADItional capillary electrophoresis (CE) methods.

Superior analysis of challenging samples

Cases are often complicated and sometimes unresolved due to the presence of highly degraded DNA, low quality DNA, or complex DNA mixtures. The ForenSeq DNA Signature Prep Kit includes a large number of Markers, many of which are highly polymorphic.

These additional Markers, coupled with the inherent sensitivity of Illumina chemistry, help detect minor components that might go undetected by conventional STR and CE analysis.

Specifications:

Assay Time ~6.5 hours

Hands-On Time ~3.5 hours

Input Quantity 10 ng high-quality genomic DNA; 10 – 100 ng FFPE DNA (depending on QC results)

Mechanism of Action Probe hybridization, extension-ligation, and PCR

Multiplexing 1–96

Content Specifications Design custom probes to sequence genomic regions of interest. Content range: 4 – 650 kb

System Compatibility NovaSeq 5000,HiSeq 2000,HiScanSQ,MiSeq FGx

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Short Tandem Repeats (STRs)

Specialized Sample Types Low Input

Technology Sequencing

Species Category Human

Method Amplicon Sequencing,Targeted DNA Sequencing

ForenSeq DNA Signature Prep Kit Workflow:

The ForenSeq DNA Signature Prep Kit is part of a fully integrated, sample-to-answer solution, including library preparation, DNA sequencing platform, and data analysis software specifically designed for forensic genomics.

ForenSeq DNA Signature Prep Kit Forensic Loci and Investigative Workflow:

With ~200 genetic Markers in a single workflow, the MiSeq FGx System offers the most comprehensive multiplex of STRs and SNPs and the most straightforward path to human identification.

15,500.00 盒

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